Instrumental Analytical Chemistry

1. Describe, select and recognize which analytical method could be use for a specific analyte and a specific sample in a defined military environment.
2. Compare, interpret and explain the results obtained from the analytical process.
3. Apply, adapt, solve and use their knowledge to unforseen military requirements to solve, by then, an unknown problem.
4. Identify and analyze the problem, and show the end-user information obtained by analytical process.
5. Compare and connect existing ideas, provide a new solution, and propose a plan to solve military problems.
6. Evaluate, compare, select, recommend and conclude what is the best analytical method for a given real problem.

Ex chatedra, Power Point presentation.

Laboratory exercise in Instrumental laboratory.

1. Lectures: Familiarization with the program, laboratory exercises instructions. Analytical methods introduction. Exercises: UV-VIS spectrometry and turbidimetry - method of external standards (calibration chart).
2. Lectures: Types of analytical signal, the basic components of instruments, instrumentation development, classification of instrumental methods. Exercises: UV-VIS spectrometry and turbidimetry - method of external standards (calibration chart).
3. Lectures: Spectrometry, history of spectroscopic techniques, fundamentals of spectrometry, classification of spectrometry - molecular spectroscopy and atomic spectrometry. Exercises: UV-VIS spectrometry and turbidimetry - method of external standards (calibration chart).
4. Lectures: Classification of spectrometry due to the interaction of the sample with the energy absorption, induced absorption, emission, polarization EMR, scattering, the ratio of the mass and charge. Exercises: AA spectrometry - a method of standard addition.
5. Lectures: Spectrometry methods of electron and ion radiation, mass spectrometry. Exercises: AA spectrometry - a method of standard addition.
6. Lectures: Electroanalytical methods, history of electroanalytical methods, basics of electroanalytical methods. Exercises: Potentiometry: potentiometric titration. Data processing of potentiometric titration.
7. Lectures: Classification of electroanalytical methods, electrochemical cell. Exercises: Potentiometry: potentiometric titration. Data processing of potentiometric titration.
8. Lectures: Potentiometric and conductiometric techniques. Exercises: Direct potentiometry.
9. Lectures: Electrogravimetry and coulometry techniques Exercises: Direct potentiometry.
10. Lectures: Voltammetric and amperometric techniques. Exercises: Conductometry: conductometric titration.
11. Lectures: Thermal analysis techniques Exercises: Conductometry: conductometric titration.
12. Lectures: Instrumental separation methods, chromatography - introduction to chromatography, gas chromatography. Exercises: Chromatography: liquid chromatography (HPLC-DAD) - a method of internal standards.
13. Lectures: Supercritical fluid chromatography, liquid chromatography, planar chromatography, ion chromatography, size-exclusion chromatography. Exercises: Chromatography: liquid chromatography (HPLC-DAD) - a method of internal standards.
14. Lectures: Electrophoresis, capillary electrophoresis. Exercises: Compensation exercises.
15. Lectures: Students’ essays presentation. Exercises: Compensation exercises.